Mapping file error and warning details.
Notes for interpreting this report:
- Errors will be listed in red, warnings in yellow.
- Mouse over an error or warning in a cell for more details.
- Errors in the header row may mask other errors, so these should be corrected first.
- Modifications to your mapping file to fix certain issues may result in different errors. You should run validate_mapping_file.py until no errors (nor warnings, ideally) are found.
Some general rules about formatting mapping files (see here for additional details):
- Header characters should only contain alphanumeric and _ characters only.
- Valid characters for SampleID fields are alphanumeric and . only.
- Other fields allow alphanumeric and +-%./ :,;_ characters.
General issues with your mapping file (i.e., those that do not pertain to a particular cell) will be listed here, if any:
SampleID | BarcodeSequence | LinkerPrimerSequence | center_name | experiment_design_description | instrument_model | library_construction_protocol | platform | collection_timestamp | day | days_since_experiment_start | dna_extracted | host_subject_id | latitude | longitude | month | physical_specimen_location | physical_specimen_remaining | reported_antibiotic_usage | sample_type | scientific_name | taxon_id | year | Description |
10589.L1S8 | AGCTGACTAGTC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 1 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | gut | human gut metagenome | 40817 | 2008 | 1_Fece_10_28_2008
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10589.L1S140 | ATGGCAGCTCTA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 2 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | gut | human gut metagenome | 40817 | 2008 | 2_Fece_10_28_2008
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10589.L1S57 | ACACACTATGGC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 1 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | gut | human gut metagenome | 40817 | 2009 | 1_Fece_1_20_2009
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10589.L1S208 | CTGAGATACGCG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 2 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | gut | human gut metagenome | 40817 | 2009 | 2_Fece_1_20_2009
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10589.L1S76 | ACTACGTGTGGT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 2/17/2009 | 17 | 112 | Yes | 1 | 40.015 | -105.271 | 2 | UCSDMI | Yes | No | gut | human gut metagenome | 40817 | 2009 | 1_Fece_2_17_2009
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10589.L1S105 | AGTGCGATGCGT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 1 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | gut | human gut metagenome | 40817 | 2009 | 1_Fece_3_17_2009
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10589.L1S257 | CCGACTGAGATG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 2 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | gut | human gut metagenome | 40817 | 2009 | 2_Fece_3_17_2009
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10589.L1S281 | CCTCTCGTGATC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 2 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | gut | human gut metagenome | 40817 | 2009 | 2_Fece_4_14_2009
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10589.L2S240 | CATATCGCAGTT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 2 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | left palm | human skin metagenome | 539655 | 2008 | 2_L_Palm_10_28_2008
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10589.L2S155 | ACGATGCGACCA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 1 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 1_L_Palm_1_20_2009
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10589.L2S309 | CGTGCATTATCA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 2 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 2_L_Palm_1_20_2009
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10589.L2S175 | AGCTATCCACGA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 2/17/2009 | 17 | 112 | Yes | 1 | 40.015 | -105.271 | 2 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 1_L_Palm_2_17_2009
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10589.L2S204 | ATGCAGCTCAGT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 1 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 1_L_Palm_3_17_2009
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10589.L2S357 | CTAACGCAGTCA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 2 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 2_L_Palm_3_17_2009
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10589.L2S222 | CACGTGACATGT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 1 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 1_L_Palm_4_14_2009
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10589.L2S382 | CTCAATGACTCA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 2 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | left palm | human skin metagenome | 539655 | 2009 | 2_L_Palm_4_14_2009
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10589.L3S242 | ACAGTTGCGCGA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 1 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | right palm | human skin metagenome | 539655 | 2008 | 1_R_Palm_10_28_2008
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10589.L3S378 | ATCGATCTGTGG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 2 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | right palm | human skin metagenome | 539655 | 2008 | 2_R_Palm_10_28_2008
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10589.L3S294 | CACGACAGGCTA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 1 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 1_R_Palm_1_20_2009
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10589.L4S63 | CTCGTGGAGTAG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 2 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 2_R_Palm_1_20_2009
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10589.L3S313 | AGTGTCACGGTG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 2/17/2009 | 17 | 112 | Yes | 1 | 40.015 | -105.271 | 2 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 1_R_Palm_2_17_2009
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10589.L3S341 | CAAGTGAGAGAG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 1 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 1_R_Palm_3_17_2009
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10589.L4S112 | GCGTTACACACA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 2 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 2_R_Palm_3_17_2009
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10589.L3S360 | CATCGTATCAAC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 1 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 1_R_Palm_4_14_2009
|
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10589.L4S137 | GAACTGTATCTC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 2 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | right palm | human skin metagenome | 539655 | 2009 | 2_R_Palm_4_14_2009
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10589.L5S104 | CAGTGTCAGGAC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 1 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | tongue | human oral metagenome | 447426 | 2008 | 1_Tong_10_28_2008
|
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10589.L5S240 | CTGGACTCATAG | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 10/28/2008 | 28 | 0 | Yes | 2 | 40.015 | -105.271 | 10 | UCSDMI | Yes | Yes | tongue | human oral metagenome | 447426 | 2008 | 2_Tong_10_28_2008
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10589.L5S155 | ATCTTAGACTGC | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 1 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 1_Tong_1_20_2009
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10589.L6S20 | GAGGCTCATCAT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 1/20/2009 | 20 | 84 | Yes | 2 | 40.015 | -105.271 | 1 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 2_Tong_1_20_2009
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10589.L5S174 | CAGACATTGCGT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 2/17/2009 | 17 | 112 | Yes | 1 | 40.015 | -105.271 | 2 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 1_Tong_2_17_2009
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10589.L5S203 | CGATGCACCAGA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 1 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 1_Tong_3_17_2009
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10589.L6S68 | GATACGTCCTGA | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 3/17/2009 | 17 | 140 | Yes | 2 | 40.015 | -105.271 | 3 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 2_Tong_3_17_2009
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10589.L5S222 | CTAGAGACTCTT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 1 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 1_Tong_4_14_2009
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10589.L6S93 | GATTAGCACTCT | GTGCCAGCMGCCGCGGTAA | CCME | Sample collection and DNA isolation were performed as described in Costello et al. [6]; and PCR, sequencing, and quality filtering of reads were performed as described in Caporaso et al. [14]. Samples were not collected on days 422 through 437. To facilitate massively parallel sequencing (1967 samples), barcodes were reused across six lanes in a single Illumina GAIIx, with 374, 372, 364, 271, 265, and 323 samples in lanes one through six, respectively (differing from Caporaso et al. [14], where samples were pooled and run over seven lanes). Sixteen samples were ultimately excluded from the analysis as fourteen samples were identified as potentially mislabeled (discussed below), and the barcodes for two samples were not found in the sequencing output, likely indicating a problem with amplification for those two samples. | Illumina HiSeq 2000 | EMP V4 515f,806rbc protocol | Illumina | 4/14/2009 | 14 | 168 | Yes | 2 | 40.015 | -105.271 | 4 | UCSDMI | Yes | No | tongue | human oral metagenome | 447426 | 2009 | 2_Tong_4_14_2009
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