This text describes how the "healthy subset" used for the main American Gut manuscript (in preparation) is defined. The definition of this healthy subset may not be appropriate for all analyses (i.e., it might be too restrictive, or not restrictive enough), so you are encouraged to define your subsets accordingly to the questions you are asking from the American Gut dataset. We filtered the cohort to conserve samples based on the following criteria: adults 20-69 years of age with BMIs ranging between 18.5 and 30, and no self-reported history of inflammatory bowel disease, diabetes, or antibiotic use in the past year.